Dylight 550 vs pe

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August undefined, 2024

WebH9 human embryonic stem (ES) cells were stained with either PE Mouse Anti-SSEA-4 (solid line) or PE mouse IgG3 (A112-3) isotype control (catalog number 559926, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System.

A Guide to Fluorochromes - Abcam

WebDyLight 550 561 562 576 FL3 FL2 FL2 DyLight 550 PE 488, 561 496, 565 578 FL3 FL3 FL2 FL2 FL2 FL2 PE ... PE-Cy5 488, 561 496, 565 670 FL4 FL4 FL4 FL4 FL3 FL2 PE … WebTable of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. simply med lemoore

DyLight™ 550 Maleimide - Thermo Fisher Scientific

WebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … WebDyLight 550 562 576 4 PE PE 496/546 578 5 DyLight 550 hFab Rhodamine* 530 580 3 StarBright Violet 610 402 607 4 BD Horizon Brilliant Violet 605, Super Bright 600 ... APC-Cy7, DyLight 750 PE-Cy7 Infrared : 496, 546 785 4 PE-Alexa Fluor 750 APC-Cy7 Infrared : … Web62269 DyLight 550-2xPEG NHS Ester, 1mg. 62274 DyLight 650-4xPEG NHS Ester, 1mg. 46601 DyLight 680-4xPEG NHS Ester, 1mg. 62277 DyLight 755-4xPEG NHS Ester, … raytheon technologies employee store

S3e Cell Sorter Laser Configuration Guide - Bio-Rad Laboratories

WebCatalog number: 62262. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight … WebDyLight™ 550 has a bright orange to red fluorescence rhodamine derivative dye. This dye can be used to replace Alexa555, TRITC and Cy3. DyLight™ 550 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross ... raytheon technologies earnings callWebBoster Bio Anti-Human CD2AP DyLight® 550 conjugated Antibody catalog # A01756-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human. … raytheon technologies ethics

"WebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 550 fluorochrome? The excitation is 562nm and the emission is 576nm. As a comparison, the excitation of DyLight® … " - Dylight 550 vs pe

Dylight 550 vs pe

DyLight™ Antibody Labeling Kits - Thermo Fisher Scientific

WebTo use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser … WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View …

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WebBackground. CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface … WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View …

WebHiLyte FluorTM 555 550 566 5-TAMRA 541 568 Alexa Fluor®546 556 573 532 DyLight®550 562 576 Phycoerythrin (PE) 496,566 576 488 Tetramethyl Rhodamine Isothiocyanate (TRITC) 557 576 EviTag TM quantum dots-Birch Yellow 560 580 Cy3.5® … WebPE (37) PE / Atto594 (166) PE / Cy5.5 (166) PE / Cy7 (166) PerCP (12) ... To understand more about direct vs. indirect detection, ... DyLight 550: NBP1-72732R: DyLight 594: …

WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. DyLight dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio ...

Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. …

WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. ... with the exception of RPE and PE tandem dyes. For more details on the recommendations … simply mediterranean menuWebThermo Scientific DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing … raytheon technologies everett waWebDec 22, 2014 · The high fluorescence part of the emission spectra typically spans about 50 nm more than for corresponding Alexa dye, i.e. 125 nm instead of 75 nm. With three colours you get approx. 375 nm of ... simplymeds codeWebLDL is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extra-hepatic tissues through receptor mediated endocytosis triggered by apoB-100-LDL receptor interaction. The internalized LDL particle is transported to lysosomes where it is degraded to free ... simplymed pro ltdWebDyLight 550 562 576 4 PE* 496, 546 578 5 APC 650 661 4 Alexa Fluor 647 650 665 4 DyLight 650 654 673 4 PerCP 490 675 2 Alexa Fluor 700 Infrared 702 723 2 * PE is the … raytheon technologies executivesWebView Mouse Monoclonal anti-HLA DRB1 Antibody (SPM423) [PE] (NBP3-11552PE). Validated Applications: Flow. Validated Species: Human, Monkey. simply meds discount codeWebJan 26, 2012 · IHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. simplymeds discount code