Ime intron plant
Witryna31 sty 2008 · Introns play a very important role in regulating gene expression in eukaryotes. In plants, many introns enhance gene expression, and the effect of intron-mediated enhancement (IME) of gene expression is reportedly often more profound in monocots than in dicots. To further gain insight of IME in monocot plants, we … Witryna24 wrz 2024 · To determine if sequences involved in IME can stimulate ... L. E. Separable whorl-specific expression and negative regulation by enhancer elements within the AGAMOUS second intron. Plant ...
Ime intron plant
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Witryna12 mar 2024 · ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription … Witryna3 kwi 2024 · Previously generated transgenic TRP1:GUS plants with the first intron of UBQ10 at three additional locations , designated herein as positions 0, −1, and −2, …
Witryna1 paź 2024 · In many eukaryotes, including mammals, plants, yeast, and insects, introns can increase gene expression without functioning as a binding site for transcription factors. This phenomenon was termed ‘intron-mediated enhancement’. ... The role of splicing in IME and the significance of intron position relative to the sites of … WitrynaIntrons of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5′UTR sequence of some rice β-tubulin genes can sustain IME in …
Witryna24 cze 2024 · Consistent with plant and mammalian studies, we determined a single, natural or synthetic, 5′-intron is sufficient for the full IME effect conferred by three … Witryna28 kwi 2008 · However, the mechanism underlying intron-mediated enhancement (IME) in plants is largely unknown. Rose et al. (pages 543–551) adopted a bioinformatics …
WitrynaIME; however, a large internal deletion of the first intron of the maize Adh gene reduces splicing and con sequently IME (Luehrsen and Walbot 1994b). A point mutation at the 5'- or 3'- splice site of the maize Hsp82 intron impaired splicing, suggesting that IME requires intron splicing (Sinibaldi and Mettler 1992). In contrast,
Witryna19 maj 2006 · However, the intrinsic determinants of 5'UTR IME in plants, especially the role of any sequence motifs within the intron, remain poorly defined. ... Luehrsen K, Walbot V: Addition of A- and U-rich sequence increases the splicing efficiency of a deleted form of a maize intron. Plant Mol Biol. 1994, 24: 449-463. … crystalline phaneriticWitryna20 wrz 2010 · In plants, Intron Mediated Enhancement (IME) of gene expression has progressively been found to be a much more common event than originally suspected. The list of enhancing introns that have been experimentally tested includes 40 different members at least, yet the proportion of plant introns endowed with enhancing … dwp simplyWitryna22 paź 2024 · Plant biotechnology traits provide a means to increase crop yields, manage weeds and pests, and sustainably contribute to addressing the needs of a growing population. One of the key challenges in developing new traits for plant biotechnology is the availability of expression elements for efficacious and predictable … crystalline penicillin injectionWitryna2 dni temu · Introduction. Recent studies showed that alternative splicing (AS) is highly pervasive in plants (Reddy et al., 2013), and introns of more than 60% genes undergo this process (Syed et al., 2012).AS plays a key role in the regulation of gene regulatory networks to help plants adapt to environmental fluctuations (Syed et al., … dwps loginWitryna1 cze 2008 · Introns mediate complex gene regulation via alternative splicing; they may act in cis as expression enhancers through IME (intron-mediated enhancement of gene expression) and in trans as negative regulators through the generation of intronic microRNA. Furthermore, some introns also contain promoter sequences for … dwp sloughSince it has been shown that specifically the first intron bears the capacity to influence expression of the gene it is part of, the set of Arabidopsis introns was split into two sets, one with only the first introns, i.e. the 5′-most, of each gene, and another for all remaining introns, termed “other introns”. … Zobacz więcej For identifying candidate intron motifs associated with IME, a k-mer-based strategy similar to IMEter was applied, with additionally utilizing conservation and relative position in introns as informative criteria, similarly … Zobacz więcej The set of hexamers contained sequence-related hexamer sequences, which may be equivalent in function or part of a larger consensus motif, … Zobacz więcej To further evaluate the newly identified motifs, they were compared to IMEter, the most commonly used tool for identifying potentially IME … Zobacz więcej dwps mhyWitryna6 sty 2024 · However, the mechanism behind this so called intron-mediated enhancement (IME) is still largely unknown. This review highlights the complexity of … crystalline perfection loba