Incipient cytorrhysis assay
WebSep 6, 2012 · Appressorial turgor pressure was determined by incipient-cytorrhysis assay reported previously ( Howard et al. 1991 ). The mycelia were placed onto artificial cellophane maintained on water agar plates and incubated at 25 °C, and the samples were microscopically observed at intervals. WebJul 5, 2016 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–4 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspensions (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature.
Incipient cytorrhysis assay
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WebAug 20, 2024 · To elucidate the defect in appressorium penetration caused by myriocin/FB1, we measured the internal turgor pressure within appressoria using an … WebAug 3, 2024 · Glycogen staining and cytorrhysis assays Appressorium turgor was measured using an incipient cytorrhysis assay. Twenty microlitres of the conidial suspension (5 × 10 4 spores/ml) was placed on hydrophobic coverslips and incubated in a humid chamber for 24 h at room temperature.
WebOct 3, 2024 · Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross … WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying …
WebAug 4, 2014 · Yeast two hybrid (Y2H) assays were carried out to clarify the interacting network of SNF1 pathway in M. oryzae. The results provided evidence that MoSnf1, MoSip2, and MoSnf4 physically interacted with each other, hinting the importance of the complex integrity ( Figure 1B ). WebFeb 18, 2024 · Considering that the germinating and appressorial formation were normal in the Mohat1 mutant (Table S1), we measured the internal concentration of solute and the turgor pressure within appressoria by the incipient cytorrhysis (cell collapse) assay using 1–4 molar of glycerol [ 45 ].
WebAug 3, 2024 · Glycogen staining and cytorrhysis assays Appressorium turgor was measured using an incipient cytorrhysis assay. Twenty microlitres of the conidial suspension (5 × …
WebNational Center for Biotechnology Information canada archery storeWebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of cytorrhysis determined ( de Jong et al. 1997 ). canada aquarium fish onlineWebIncipient cytorrhysis determined the turgor pressure in appressoria of P.pachyrhizi to be equivalent to 5.13MPa. MZM data revealed that osmotically active sugar alcohols only ... uredospores for the germination assay on glass slides, older ure-dospores were removed from the leaves by shaking 1d before spore harvest. The M.oryzae isolate TH6772 ... canada archery bear huntsWebAug 29, 2016 · Appressoria formed at 24 h post-incubation were used in an incipient cytorrhysis assay to measure appressorial turgor. The experiments were replicated three times and more than 200 appressoria were counted for … canada apply for health numberWebJan 15, 2003 · Appressorium turgor can be measured using an incipient cytorrhysis assay which uses hyperosmotic concentrations of a solute to collapse appressoria, thereby … canada archery teamWebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface. canada archery supplyWebDec 20, 2011 · The appressorial turgor pressure was determined using the incipient cytorrhysis assay, as reported previously ( Howard et al. 1991 ). Each assay was completely independent and replicated at least three times. Plant infection assays fishearea