Phosphinothricin acetyltransferase gene
WebCloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli Gene 63 65-74 1988 Streptomyces viridochromogenes, Streptomyces viridochromogenes Tue494 3290054. brenda. 673843. Webmechanism of Agrobacterium-mediated gene transfer has been widely utilized to genetically modify mono-cots and dicots (Hansen and Wright, 1999; Fukuoka et al., 2000). Agrobacterium tumefaciensis a soil-borne pathogen that has the capacity to infect plants and transfer a segment of DNA (T-DNA) from its tumor-inducing (Ti) plasmid to the plant …
Phosphinothricin acetyltransferase gene
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WebJan 1, 1995 · Phosphinothricin is an inhibitor of glutamine synthetase, which plays a central role in the assimilation of ammonia and in the regulation of the nitrogen metabolism in plants. 9, 10 It is the only enzyme that detoxifies ammonia produced during nitrate reduction, photorespiration, and amino acid degradation in plant cells. WebApr 3, 2024 · Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance.To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with …
WebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ...
WebNational Center for Biotechnology Information WebThis gene encodes neomycin phosphotransferase, which confers resistance to antibiotics such as kanamycin, gentamicin A, neomycin, and others when expressed in plants. The hpt gene, also isolated from E. coli, codes for hygromycin transferase and confers resistance …
WebPhosphinothricin N-acetyltransferase. Gene. bar. Status. UniProtKB reviewed (Swiss-Prot) Organism. Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) Amino acids. 171. Protein existence. Evidence at protein level. Annotation score. 3/5.
WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … dhaka to bangkok cheapest flightWebIn this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. ... GmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) ... dhaka to boston flightsWebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector … cider shopping australiaWebphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … cider shirtWebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat dhaka to chapai nawabganj bus ticket priceWebl -Phosphinothricin ( l -Pt)-resistant plants were constructed by introducing a modified phosphinothricin- N -acetyl-transferase gene ( pat) via Agrobacterium -mediated gene … cider sweater dressWebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … dhaka to cape town